Functional Analysis of a Subset of Canine Olfactory Receptor Genes
Identifieur interne : 002B97 ( Main/Exploration ); précédent : 002B96; suivant : 002B98Functional Analysis of a Subset of Canine Olfactory Receptor Genes
Auteurs : Naima Benbernou [France] ; Sandrine Tacher [France] ; Stéphanie Robin [France] ; Michaelle Rakotomanga [France] ; Fabrice Senger [France] ; Francis Galibert [France]Source :
- Journal of Heredity [ 1465-7333 ] ; 2007.
Abstract
In this paper, we explored the level complexity of the combinatorial olfactory code that allows mammals with a repertoire of about thousand putatively active olfactory receptors encoded in their genomes to recognize and identify a much larger repertoire of odorant molecules. To that end, we cloned 38 canine OR genes belonging to the same OR gene family and transiently expressed them in a subclone of embryonic human kidney cells (HEK293) permanently expressing the G(olf) subunit. Using a Ca2+ imaging approach, we established for example that as many as 26 out of the 38 cloned OR elicited a Ca2+ response when exposed to octanal, whereas 10 responded to nonanal, other aldehydes providing intermediate responses. Altogether, these results demonstrated that the combinatorial code is quite complex in support to the highly developed sense of olfaction demonstrated by dogs.
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DOI: 10.1093/jhered/esm054
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Senger: UMR 6026, Faculté des sciences, Beaulieu, Rennes</wicri:regionArea><placeName><region type="region">Région Bretagne</region><region type="old region">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Robin, Stephanie" sort="Robin, Stephanie" uniqKey="Robin S" first="Stéphanie" last="Robin">Stéphanie Robin</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>From the Laboratory of Genetic and Development, CNRS UMR 6061, Institut de Génétique et Développement de Rennes, Faculté de Médecine, Rennes, F-35043 France (Benbernou, Tacher, Robin, Rakotomanga, Senger, and Galibert) Present address of F. 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Senger: UMR 6026, Faculté des sciences, Beaulieu, Rennes</wicri:regionArea><placeName><region type="region">Région Bretagne</region><region type="old region">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Heredity</title><title level="j" type="issue">Symposium Issue: Third International Conference-Advances in Canine and Feline Genomics School of Veterinary Medicine, University of California, Davis, CA, August 3-5, 2006.</title><idno type="eISSN">1465-7333</idno><idno type="ISSN">0022-1503</idno><imprint><publisher>Oxford University Press</publisher><date>2007</date><date type="e-published">2007</date><biblScope unit="vol">98</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="500">500</biblScope><biblScope unit="page" to="505">505</biblScope></imprint><idno type="ISSN">0022-1503</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1503</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">In this paper, we explored the level complexity of the combinatorial olfactory code that allows mammals with a repertoire of about thousand putatively active olfactory receptors encoded in their genomes to recognize and identify a much larger repertoire of odorant molecules. To that end, we cloned 38 canine OR genes belonging to the same OR gene family and transiently expressed them in a subclone of embryonic human kidney cells (HEK293) permanently expressing the G(olf) subunit. Using a Ca2+ imaging approach, we established for example that as many as 26 out of the 38 cloned OR elicited a Ca2+ response when exposed to octanal, whereas 10 responded to nonanal, other aldehydes providing intermediate responses. Altogether, these results demonstrated that the combinatorial code is quite complex in support to the highly developed sense of olfaction demonstrated by dogs.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Région Bretagne</li></region><settlement><li>Rennes</li></settlement></list><tree><country name="France"><region name="Région Bretagne"><name sortKey="Benbernou, Naima" sort="Benbernou, Naima" uniqKey="Benbernou N" first="Naima" last="Benbernou">Naima Benbernou</name></region><name sortKey="Galibert, Francis" sort="Galibert, Francis" uniqKey="Galibert F" first="Francis" last="Galibert">Francis Galibert</name><name sortKey="Galibert, Francis" sort="Galibert, Francis" uniqKey="Galibert F" first="Francis" last="Galibert">Francis Galibert</name><name sortKey="Rakotomanga, Michaelle" sort="Rakotomanga, Michaelle" uniqKey="Rakotomanga M" first="Michaelle" last="Rakotomanga">Michaelle Rakotomanga</name><name sortKey="Robin, Stephanie" sort="Robin, Stephanie" uniqKey="Robin S" first="Stéphanie" last="Robin">Stéphanie Robin</name><name sortKey="Senger, Fabrice" sort="Senger, Fabrice" uniqKey="Senger F" first="Fabrice" last="Senger">Fabrice Senger</name><name sortKey="Tacher, Sandrine" sort="Tacher, Sandrine" uniqKey="Tacher S" first="Sandrine" last="Tacher">Sandrine Tacher</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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